Products
Basal Media

Basal Media

Cell culture media

Ajinomoto Genexine’s CELLiST BASAL Media for Chinese Hamster Ovary (CHO) cell lines are developed based on Ajinomoto’s strong capabilities in amino acid technology and over 30 years of experience in the cell culture media business.
CELLiST Media are high-performance, chemically-defined media, free from any animal-derived ingredients.

CELLiST BASAL Media are all Chemically Defined Media, optimized for Chinese Hamster Ovary (CHO) cells cultures and the production of recombinant proteins.
CELLiST BASAL Media are developed based on Ajinomoto's capabilities in cell culture media development and know-how of amino acids science. CELLiST BASAL Media can be applied to various CHO cell lines, including CHO-K1, CHO-S, CHO-DG44 and CHO-DXB11.

Features

  • Chemically formulated and free from animal-derived ingredients.
  • Contains high-quality amino acids based on the Ajinomoto's long-standing amino acid research capabilities and expertise.
  • Suitable for most CHO cell lines including CHO-K1, CHO-S, CHO-DG44 and CHO-DXB11.
  • Packaging form can be adjusted according to customer’s requirements.

Specifications

Model No. BASAL3
TYPE Powder
Concentration 27.0g / L
With Additives D-Glucose
Without Additives L-Glutamine · Sodium hydrogen carbonate Thymidine Hypoxanthine · Poloxamer · Insulin and other growth factors
Storage Condition 2℃ to 8℃, dark and dry
Shelf Life 36 months
Item Description Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Related Documents

Performance

CHO-K1 cell line used:
Comparison data between other companies’ media and CELLiST media.

A. Viable Cell Density
B. Normalized lgG Titer

CHO-DG-44 cell line used:
Fed-batch culture of BASAL3 and FEED2

A. Viable cell density
B. lgG titer(Day14)
“BASAL3+FEED2” and other eight commercial CD media sets were evaluated in fed-batch culture. 4% of FEED2(CELLiST) was added on days 4. 7. 9 and 11. Feeding manner for other commercial media sets followed each manufacturer’s recommendation. As a result. CELLiST showed the highest VCD and lgG titer compared to other media.

CHO-S cell Line used:
Fed-batch culture of BASAL3/10 and FEED2

A. Viable cell density
B. lgG titer(Day14)
Using CHO-S cell line, combinations of our CELLiST and other five commercial CD media sets were compared in fed-batch culture. 4% of FEED2 (CELLiST) was added on days 4, 6, 8 and 12. Feeding manner for other commercial media sets followed each manufacturer’s recommendation. Consequently, CELLiST showed higher igG titer compared to other media.

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