Products

Basal Media

Cell culture media

Ajinomoto CELLiST Korea’s CELLiST BASAL Media for Chinese Hamster Ovary (CHO) cell lines are developed based on Ajinomoto’s strong capabilities in amino acid technology and over 30 years of experience in the cell culture media business.
CELLiST Media are high-performance, chemically-defined media, free from any animal-derived ingredients.

CELLiST BASAL Media are all Chemically Defined Media, optimized for Chinese Hamster Ovary (CHO) cells cultures and the production of recombinant proteins.
CELLiST BASAL Media are developed based on Ajinomoto's capabilities in cell culture media development and know-how of amino acids science. CELLiST BASAL Media can be applied to various CHO cell lines, including CHO-K1, CHO-S, CHO-DG44 and CHO-DXB11.

Features

  • Chemically formulated and free from animal-derived ingredients
  • Contains high-quality amino acids based on the Ajinomoto's long-standing amino acid research capabilities and expertise
  • Suitable for most CHO cell lines including CHO-K1, CHO-S, CHO-DG44 and CHO-DXB11
  • Packaging form can be adjusted according to customer’s requirements

Specifications

Model No. BASAL3
TYPE Powder
Concentration 27.0g / L
With Additives D-Glucose
Without Additives L-Glutamine · Sodium hydrogen carbonate · Thymidine Hypoxanthine · Poloxamer · Insulin and other growth factors
Storage Condition 2℃ to 8℃, dark and dry
Shelf Life 36 months
Item Description Powder, chemically defined and animal derived component free medium, rehydrate to 1 L

Related Documents

Performance

CHO-K1 cell line
Fed-batch culture of CELLiST BASAL3/BASAL10 with FEED2

A. Viable cell density
B. IgG Titer (Day 14)
CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against five popular commercial media brands in fed-batch culture. 6% of FEED2 was added on days 4, 6, 8, 10 and 12. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.

CHO-GS (CHOZN®) cell line
Fed-batch culture of CELLiST BASAL3/BASAL10 with FEED2

A. Viable cell density
B. IgG Titer (Day 14)
CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against six popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 7, 9, and 11. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.

CHO-DG44 cell line
Fed-batch culture of CELLiST BASAL3 with FEED2

A. Viable cell density
B. IgG Titer (Day 14)
CELLiST BASAL3+FEED2 was evaluated against eight popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 7, 9, and 11. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.

CHO-S cell line
Fed-batch culture of CELLiST BASAL3/BASAL10 with FEED2

A. Viable cell density
B. IgG Titer (Day 14)
CELLiST BASAL3+FEED2 and BASAL10+FEED2 combinations were evaluated against five popular commercial media brands in fed-batch culture. 4% of FEED2 was added on days 4, 6, 8, and 12. Feeding manner for other commercial media was followed according to each manufacturer’s recommendation.

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