Ajinomoto CELLiST Korea’s CELLiST™ Amino Supplement Media for CHO cell lines are developed based on Ajinomoto’s expertise in amino acid science and over 30 years of experience in the cell culture media business. CELLiST™ Media are high-performance, chemically-defined media, free from any animal-derived ingredients.
By using media supplements for cell culture experiments, there are many advantages for titer improvement as well as cost effectiveness.
CELLiST™ AminoSupplement help ensure the reliability and consistency of your cell culture and research results.
| Product name | Main Target | Format | Package type | Qty. |
|---|---|---|---|---|
| AminoSupplement Cys1 | Single-agent feed media users | Powder | 1 L Aluminum pouch | 6.61 g |
| 50 L Plastic Drum | 331 g | |||
| AminoSupplement Cys2 | Two-agent feed media users | Powder | 1 L Aluminum pouch | 17.0 g |
| 50 L Plastic Drum | 425 g |
Adding AminoSupplement Cys1/2 increase Cysteine availability in the media by inhibiting Cystine formation. This leads to improvement in overall productivity, as can be seen below.
Fed-batch cultures were compared using cells of CHO-DG44. FEED2 was added 5.25% on days 4, 6, 8, 10, and 12 of culture.
Fed-batch cultures were compared using cells of CHO-S. Feed media from other companies was added 6% to the basic media from other companies on culture days 4, 6, 8, 10, and 12.
Fed-batch cultures were compared using cells of CHO-S. Feed media from other companies was added 4.5% to the basic media from other companies on cultures 4, 6, 8, 10, and 12 days.
Adding AminoSupplement Cys1/Cys2 has no major impact on glycan profile and Charge Variant as follows, so you can use it without any significant change in the culture process.
Two-agent feed media requires two tanks due to instability. However, adding AminoSupplement Cys2 improves the stability, which allows it to be prepared in a single tank and save much initial & running costs.
| day | 0 | 10 | 14 | 17 | 21 | 24 | 28 | 31 | |
| Other feed media(feed A+B) w/o AminoSupplement Cys2 |
10 mL | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
|---|---|---|---|---|---|---|---|---|---|
| 40 mL | +++ | +++ | +++ | +++ | +++ | +++ | +++ | +++ | |
| Other feed media(feed A) w/ AminoSupplement Cys2 |
10 mL | — | — | — | — | — | — | — | + |
| 40 mL | — | — | — | — | — | — | — | — |
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